[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"health-study-detail:100541809":3},{"organization":4,"armGroups":7,"interventions":18,"overallOfficials":24,"centralContacts":29,"locations":38,"responsibleParty":55,"collaborators":10,"id":57,"slug":58,"hasResults":59,"nctId":60,"briefTitle":61,"officialTitle":61,"acronym":62,"eligibilityCriteria":63,"healthyVolunteers":59,"sex":64,"minAge":65,"maxAge":10,"enrollmentInfo":66,"targetDuration":10,"studyType":69,"phases":10,"briefSummary":70,"conditions":71,"keywords":73,"overallStatus":41,"whyStopped":10,"lastUpdateSubmitDate":80,"lastUpdatePostDateStruct":81,"startDateStruct":84,"completionDateStruct":86,"leadSponsor":88,"locationsCount":89},{"fullName":5,"class":6},"Mario Negri Institute for Pharmacological Research","OTHER",[8,14],{"label":9,"type":10,"description":11,"interventionNames":12},"Cases",null,"Sera from patients with biopsy-proven idiopathic MCD or FSGS who provided consent to store their samples in the certified biobank (Mario Negri Biological Resources Centre - Rare Diseases and Renal Diseases Biobank - CRB). Serum samples will be tested for the levels of anti-nephrin autoantibodies by in-house ELISA and results confirmed by commercial ELISA kits.",[13],"Diagnostic Test: In-house ELISA, and ELISA kits from \"DBA Italy\" (Abbexa).",{"label":15,"type":10,"description":16,"interventionNames":17},"Controls","Sera from patients with biopsy-proven idiopathic membranous nephropathy who provided consent to store their samples in the certified biobank (Mario Negri Biological Resources Centre - Rare Diseases and Renal Diseases Biobank - CRB). Samples will be tested for the levels of anti-nephrin autoantibodies by in-house ELISA and results confirmed by commercial ELISA kits.",[13],[19],{"type":20,"name":21,"description":22,"armGroupLabels":23,"otherNames":10},"DIAGNOSTIC_TEST","In-house ELISA, and ELISA kits from \"DBA Italy\" (Abbexa).","\"Nunc MaxiSorp\" ELISA plates will be coated with recombinant extracellular domain of human nephrin. Patient serum samples will be added in appropriate dilution. Plates will be then incubated with biotin-conjugated anti human IgG antibody followed by incubation with horseradish peroxidase (HRP)-avidin conjugate. Then tetramethylbenzidine substrate will be added, and absorbance read at 450 nm.",[9,15],[25],{"name":26,"affiliation":27,"role":28},"Giuseppe Remuzzi, MD","Istituto Di Ricerche Farmacologiche Mario Negri","STUDY_DIRECTOR",[30,35],{"name":31,"role":32,"phone":33,"phoneExt":10,"email":34},"Federica Casiraghi, PhD","CONTACT","+3903545351","federica.casiraghi@marionegri.it",{"name":36,"role":32,"phone":33,"phoneExt":10,"email":37},"Norberto Perico, MD","norberto.perico@marionegri.it",[39],{"facility":40,"status":41,"city":42,"state":43,"zip":44,"country":45,"countryCode":46,"cosmosGeoPoint":47,"geoPoint":52,"contacts":53},"Clinical Research Center for Rare Disease Aldo e Cele Daccò","RECRUITING","Ranica","BG","24020","Italy","IT",{"type":48,"coordinates":49},"Point",[50,51],9.71335,45.72328,{"lat":51,"lon":50},[54],{"name":31,"role":32,"phone":33,"phoneExt":10,"email":34},{"type":56,"investigatorFullName":10,"investigatorTitle":10,"investigatorAffiliation":10,"oldNameTitle":10,"oldOrganization":10},"SPONSOR","100541809","autoantibodies-against-nephrin-in-idiopathic-nephrotic-syndrome-100541809",false,"NCT06334692","Autoantibodies Against-nephrin in Idiopathic Nephrotic Syndrome","BLINDER","Inclusion Criteria:\n\n* Adult (\\>18 years) males and females\n* Patients with biopsy-proven idiopathic MCD or FSGS (cases)\n* Patients with biopsy-proven idiopathic membranous nephropathy (controls)\n* Patients who provided consent to store their samples in the certified CRB biobank\n\nExclusion Criteria:\n\n* Reasonable possibility of a secondary cause of NS (for cases) or MN (for controls) at time of blood collections\n* Active viral or bacterial infections at time of blood collections","ALL","18 Years",{"count":67,"type":68},100,"ESTIMATED","OBSERVATIONAL","This retrospective study is aimed at evaluating the levels of circulating anti-nephrin autoantibodies in patients with INS, including those with MCD\u002FFSGS and in patients who have experienced relapse of FSGS post-transplant, compared to those of a control group of patients with nephrotic syndrome due to primary membranous nephropathy (MN).",[72],"Nephrotic Syndrome",[74,75,76,77,78,79],"Idiopathic nephrotic syndrome","Minimal change disease","Focal segmental glomerulosclerosis","Permeability factor(s)","B cells","Nephrin autoantibodies","2025-08-27",{"date":82,"type":83},"2025-09-04","ACTUAL",{"date":85,"type":83},"2024-03-19",{"date":87,"type":68},"2028-03",{"name":5,"class":6},1]