[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"health-study-detail:100629788":3},{"organization":4,"armGroups":7,"interventions":14,"overallOfficials":11,"centralContacts":20,"locations":30,"responsibleParty":57,"collaborators":11,"id":59,"slug":60,"hasResults":61,"nctId":62,"briefTitle":63,"officialTitle":64,"acronym":65,"eligibilityCriteria":66,"healthyVolunteers":61,"sex":67,"minAge":68,"maxAge":11,"enrollmentInfo":69,"targetDuration":11,"studyType":72,"phases":73,"briefSummary":75,"conditions":76,"keywords":78,"overallStatus":81,"whyStopped":11,"lastUpdateSubmitDate":82,"lastUpdatePostDateStruct":83,"startDateStruct":86,"completionDateStruct":88,"leadSponsor":90,"locationsCount":91},{"fullName":5,"class":6},"University Hospital, Grenoble","OTHER",[8],{"label":9,"type":10,"description":11,"interventionNames":12},"Metagenomic Diagnostic Evaluation","EXPERIMENTAL",null,[13],"Diagnostic Test: Plasma Microbial Cell-Free DNA Metagenomic Sequencing",[15],{"type":16,"name":17,"description":18,"armGroupLabels":19,"otherNames":11},"DIAGNOSTIC_TEST","Plasma Microbial Cell-Free DNA Metagenomic Sequencing","A 20 mL peripheral blood sample will be collected prior to neurosurgical intervention for metagenomic next-generation sequencing (mNGS) analysis of circulating microbial cell-free DNA. Results will be compared with metagenomic analysis and conventional culture of intraoperative brain abscess specimens, which serve as the reference standard for microbiological identification.",[9],[21,26],{"name":22,"role":23,"phone":24,"phoneExt":11,"email":25},"Marion LE MARECHAL, MD,PhD","CONTACT","04 76 76 52 91","MLemarechal@chu-grenoble.fr",{"name":27,"role":23,"phone":28,"phoneExt":11,"email":29},"Saber touati, PhD","04 76 76 58 05","stouati1@chu-grenoble.fr",[31,47],{"facility":32,"status":11,"city":33,"state":11,"zip":11,"country":34,"countryCode":35,"cosmosGeoPoint":36,"geoPoint":41,"contacts":42},"CHU de Grenoble Alpes, Laboratoire de bactériologie","Grenoble","France","FR",{"type":37,"coordinates":38},"Point",[39,40],5.71479,45.17869,{"lat":40,"lon":39},[43],{"name":44,"role":23,"phone":45,"phoneExt":11,"email":46},"Yvan Caspar, PharmD; PhD","04 76 76 63 12","YCaspar@chu-grenoble.fr",{"facility":48,"status":11,"city":33,"state":11,"zip":11,"country":34,"countryCode":35,"cosmosGeoPoint":49,"geoPoint":51,"contacts":52},"CHU de Grenoble Alpes, Service de réanimation neurochirurgicale",{"type":37,"coordinates":50},[39,40],{"lat":40,"lon":39},[53],{"name":54,"role":23,"phone":55,"phoneExt":11,"email":56},"Clotilde Schilte, MD","04 76 76 66 88","CSchilte@chu-grenoble.fr",{"type":58,"investigatorFullName":11,"investigatorTitle":11,"investigatorAffiliation":11,"oldNameTitle":11,"oldOrganization":11},"SPONSOR","100629788","circulating-cell-free-dna-for-brain-abscess-diagnosis-a-pilot-study-100629788",false,"NCT07479225","Circulating Cell-Free DNA for Brain Abscess Diagnosis: A Pilot Study","Assessment of Circulating Cell-Free DNA for the Microbiological Diagnosis of Brain Abscesses: A Pilot Proof-of-Concept Study","METABCES-PoC","Inclusion Criteria:\n\n* Participant ≥ 18 years of age.\n* Participant presenting with a brain abscess requiring neurosurgical\n* Signed Informed Consent\n* Covered by health insurance\n\nExclusion Criteria:\n\n* Participant in exclusion period for another study\n* Participant referred to in articles L1121-5 to L1121-8 of the CSP (corresponding to all protected persons : pregnant woman, parturient, breastfeeding mother, person deprived of liberty by judicial or administrative decision, persons undergoing psychiatric care under articles L.3212-1 and L.3213-1 who do not fall under article L.1121-8, persons admitted to a healthcare or social institution for purposes other than research, minors, person under legal protection or unable to express consent).\n* Staff members with a hierarchical relationship to the principal investigator","ALL","18 Years",{"count":70,"type":71},15,"ESTIMATED","INTERVENTIONAL",[74],"NA","Brain abscess is a severe intracranial infection associated with significant morbidity and mortality. Standard management combines neurosurgical intervention, when feasible, with prolonged intravenous antibiotic therapy. Neurosurgical procedures, such as surgical drainage or stereotactic aspiration, play a key role in reducing intracranial pressure, decreasing the infectious burden, and obtaining samples for microbiological identification.\n\nHowever, neurosurgical intervention is not always possible due to technical limitations or patien related contrindications. In these situations, microbiological documentation becomes particularly challenging. Conventional diagnostic methods have limited sensitivity, with blood cultures and lumbar puncture yielding positive results in only about 25% of brain abscess cases.\n\nRecent advances in infectious disease diagnostics have introduced metagenomic approaches that may improve pathogen detection. Studies have shown that metagenomic analysis of operative samples can provide more comprehensive microbiological documentation than conventional culture-based methods. In addition, next-generation sequencing (NGS) of circulating microbial cell-free DNA in blood enables the detection of short microbial DNA fragments with a short half-life, reflecting active infection. This technology has already demonstrated promising results in clinical situations where microbiological documentation is difficult, such as febrile neutropenia. The present study aims to evaluate the performance of this approach in patients with brain abscess.",[77],"Brain Abscess",[77,79,80],"Cell-Free DNA","Metagenomics","NOT_YET_RECRUITING","2026-03-12",{"date":84,"type":85},"2026-03-18","ACTUAL",{"date":87,"type":71},"2026-04-01",{"date":89,"type":71},"2028-03-31",{"name":5,"class":6},2]