[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"health-study-detail:100599757":3},{"organization":4,"armGroups":7,"interventions":19,"overallOfficials":32,"centralContacts":37,"locations":48,"responsibleParty":68,"collaborators":42,"id":70,"slug":71,"hasResults":72,"nctId":73,"briefTitle":74,"officialTitle":75,"acronym":76,"eligibilityCriteria":77,"healthyVolunteers":72,"sex":78,"minAge":79,"maxAge":42,"enrollmentInfo":80,"targetDuration":42,"studyType":83,"phases":84,"briefSummary":86,"conditions":87,"keywords":42,"overallStatus":51,"whyStopped":42,"lastUpdateSubmitDate":91,"lastUpdatePostDateStruct":92,"startDateStruct":95,"completionDateStruct":97,"leadSponsor":99,"locationsCount":100},{"fullName":5,"class":6},"Mỹ Đức Hospital","OTHER",[8,14],{"label":9,"type":10,"description":11,"interventionNames":12},"Single-step thawing protocol","EXPERIMENTAL","For the warming phase, vitrified blastocysts are exposed to the thawing solution of a commercial embryo thawing kit (Irvine Scientific Inc., USA) at 37°C for one minute. Immediately following this, embryos will be rinsed through a 35mm diameter dish of 2ml of pre-equilibrated thawing solution before being placed in culture media in the incubator for at least 2 hours before transfer.",[13],"Procedure: Single-step warming protocol by thawing solution only",{"label":15,"type":10,"description":16,"interventionNames":17},"Multi-step thawing protocol","For the Multi-step (MS) protocol, thawing kits were equilibrated overnight in a 37°C incubator. Warming procedures utilized the kits (Cryotech RtU, Japan). To remove the cryoprotectants, blastocysts were warmed, and cryoprotectants were diluted in a three-step process.\n\nThe warming process starts with the exposure of blastocysts to thaw solution (TS) with 1M trehalose for one minute, set at a temperature to ensure 37°C. Subsequently, blastocysts will be transferred to a second well containing dilution solution (DS) 0.5M trehalose for a two-minute rinse at room temperature. This is followed by two additional three-minute and 30 seconds rinses in the wash solution (WS) at room temperature. The timeline for standard warming of blastocysts requires a total of 6.5 min. After thawing, embryo will be placed in the incubator at least 2 hours before transfer.",[18],"Procedure: Standard warming protocol",[20,26],{"type":21,"name":22,"description":23,"armGroupLabels":24,"otherNames":25},"PROCEDURE","Single-step warming protocol by thawing solution only","Potentially eligible patients' vitrified blastocysts will be thawed by a single-step thawing protocol. For the warming phase, vitrified blastocysts are exposed to the thawing solution of a commercial embryo thawing kit (Irvine Scientific Inc., USA) at 37°C for one minute. Immediately following this, embryos will be rinsed in a 35mm diameter dish of 2ml of pre-equilibrated thawing solution before being placed in culture media in the incubator for at least 2 hours before transfer.",[9],[9],{"type":21,"name":27,"description":28,"armGroupLabels":29,"otherNames":30},"Standard warming protocol","For the MS protocol, thawing kits were equilibrated overnight in a 37°C incubator. Warming procedures utilized the kits (Cryotech RtU, Japan). To remove the cryoprotectants, blastocysts were warmed, and cryoprotectants were diluted in a three-step process.\n\nThe warming process starts with the exposure of blastocysts to thaw solution (TS) with 1M trehalose for one minute at 37°C. Subsequently, the blastocyst will be transferred to a second well containing a dilution solution (DS) of 0.5M trehalose for a two-minute rinse at room temperature. This is followed by two additional three-minute and 30-second rinses in the wash solution (WS) at room temperature. The timeline for standard warming of blastocysts requires a total of 6.5 min. After thawing, embryo will be placed in the incubator at least 2 hours before transfer.",[15],[31],"Multi-step warming protocol",[33],{"name":34,"affiliation":35,"role":36},"Lan TN Vuong, MD, PhD","University of Medicine and Pharmacy at Ho Chi Minh City","PRINCIPAL_INVESTIGATOR",[38,44],{"name":39,"role":40,"phone":41,"phoneExt":42,"email":43},"Tam TM Luu, MD","CONTACT","+84357426024",null,"tam.ltm@myduchospital.vn",{"name":45,"role":40,"phone":46,"phoneExt":42,"email":47},"Vu NA Ho, PhD","+84935843336","bsvu.hna@myduchospital.vn",[49],{"facility":50,"status":51,"city":52,"state":53,"zip":54,"country":55,"countryCode":56,"cosmosGeoPoint":57,"geoPoint":62,"contacts":63},"My Duc Hospital","RECRUITING","Ho Chi Minh City","City","7000","Vietnam","VN",{"type":58,"coordinates":59},"Point",[60,61],106.62965,10.82302,{"lat":61,"lon":60},[64],{"name":65,"role":40,"phone":66,"phoneExt":42,"email":67},"Tuong M Ho, MSc, MD","+84 90 3633377","tuongho.ivfmd@gmail.com",{"type":69,"investigatorFullName":42,"investigatorTitle":42,"investigatorAffiliation":42,"oldNameTitle":42,"oldOrganization":42},"SPONSOR","100599757","single-step-protocol-and-multi-step-warming-protocol-for-blastocyst-fet-100599757",false,"NCT07088640","Single Step Protocol and Multi-step Warming Protocol for Blastocyst FET","The Live Birth Rate Between Single and Multi-step Warming Protocol Applied in Blastocyst Vitrification: a Randomized Controlled Trial","ONESTEP","Inclusion Criteria:\n\n* Women aged from 18\n* Undergoing no more than 3 previous IVF\u002FICSI cycles\n* Had at least a single good-quality blastocyst frozen.\n* Endometrium preparation using artificial cycle\n* Agree to single blastocyst transfer\n* Not participating in any interventional studies at the same time\n\nExclusion Criteria:\n\n* Embryos from cycles after in-vitro maturation, pre-implantation genetic testing (PGT)\n* Having contraindications for exogenous hormone administration (e.g., breast cancer, thromboembolic disease)\n* Having uterine abnormalities (e.g., adenomyosis, intrauterine adhesions, unicornuate\u002F bicornuate\u002F arcuate uterus; unremoved hydrosalpinx or endometrial polyp)","FEMALE","18 Years",{"count":81,"type":82},816,"ESTIMATED","INTERVENTIONAL",[85],"NA","The multi-step thawing protocol with a reduction of non-permeable cryoprotectant concentrations to reduce osmotic shock caused by the rapid influx of water. Recent studies have shown that a simplified warming protocol by only a thawing solution gave a comparable survival rate but increased pregnancy rate, reduced patients' waiting time, and decreased the workload of embryologists.",[88,89,90],"IVF","Frozen Embryo Transfer (FET)","Embryo Thawing Protocol","2025-09-10",{"date":93,"type":94},"2025-09-12","ACTUAL",{"date":96,"type":94},"2025-08-13",{"date":98,"type":82},"2027-03-01",{"name":5,"class":6},1]