[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"health-study-detail:100571230":3},{"organization":4,"armGroups":7,"interventions":17,"overallOfficials":28,"centralContacts":32,"locations":41,"responsibleParty":57,"collaborators":23,"id":59,"slug":60,"hasResults":61,"nctId":62,"briefTitle":63,"officialTitle":64,"acronym":23,"eligibilityCriteria":65,"healthyVolunteers":61,"sex":66,"minAge":67,"maxAge":23,"enrollmentInfo":68,"targetDuration":23,"studyType":71,"phases":72,"briefSummary":74,"conditions":75,"keywords":78,"overallStatus":43,"whyStopped":23,"lastUpdateSubmitDate":81,"lastUpdatePostDateStruct":82,"startDateStruct":85,"completionDateStruct":87,"leadSponsor":89,"locationsCount":90},{"fullName":5,"class":6},"IRCCS Azienda Ospedaliero-Universitaria di Bologna","OTHER",[8,14],{"label":9,"type":6,"description":10,"interventionNames":11},"Organ donors","organ donors (legally deceased, brain death or cardiac death)",[12,13],"Diagnostic Test: histochemical and immunohistochemical analysis","Diagnostic Test: RT-PCR",{"label":15,"type":6,"description":15,"interventionNames":16},"Organ recipients",[12,13],[18,24],{"type":19,"name":20,"description":21,"armGroupLabels":22,"otherNames":23},"DIAGNOSTIC_TEST","histochemical and immunohistochemical analysis","2 µm sections will be cut from the paraffin blocks for histochemical and immunohistochemical (IHC) investigations. IHC will be performed with an automated stainer (Ventana\u002FRoche®, Ventana Medical Systems, USA) for the endothelial markers CD34, ERG, Nestin and Caveolin-1",[9,15],null,{"type":19,"name":25,"description":26,"armGroupLabels":27,"otherNames":23},"RT-PCR","Custom RT-PCR analyses will be performed manually for the transcripts (mRNA) of the ERG, Nestin, VEGFR and other genes, at the Pathology Anatomy lab hall. 18 (Prof. D'Errico). Total RNA will be extracted from the paraffin blocks with the RecoverAll™ Total Nucleic Acid Isolation kit (Invitrogen). Reverse Transcriptase will be performed with the High Capacity Reverse Transcription kit (Life Technologies), using the specific primers. Real-Time PCR will be performed with the CFX Connect Real-Time PCR Identification System (BioRad).",[9,15],[29],{"name":30,"affiliation":5,"role":31},"Francesco Vasuri, MD","PRINCIPAL_INVESTIGATOR",[33,38],{"name":34,"role":35,"phone":36,"phoneExt":23,"email":37},"Francecso Vasuri, MD","CONTACT","0512143761","francesco.vasuri2@unibo.it",{"name":39,"role":35,"phone":36,"phoneExt":23,"email":40},"Deborah Malvi, MD","deborah.malvi@aosp.bo.it",[42],{"facility":5,"status":43,"city":44,"state":23,"zip":45,"country":46,"countryCode":47,"cosmosGeoPoint":48,"geoPoint":53,"contacts":54},"RECRUITING","Bologna","40138","Italy","IT",{"type":49,"coordinates":50},"Point",[51,52],11.33875,44.49381,{"lat":52,"lon":51},[55,56],{"name":30,"role":35,"phone":36,"phoneExt":23,"email":37},{"name":30,"role":31,"phone":23,"phoneExt":23,"email":23},{"type":58,"investigatorFullName":23,"investigatorTitle":23,"investigatorAffiliation":23,"oldNameTitle":23,"oldOrganization":23},"SPONSOR","100571230","tissue-analysis-of-liver-grafts-as-a-predictor-of-transplant-outcome-100571230",false,"NCT06717542","Tissue Analysis of Liver Grafts as a Predictor of Transplant Outcome.","Tissue Analysis (Histological, Ultrastructural and Molecular) of Liver Grafts as a Predictor of Transplant Outcome.","Inclusion Criteria:\n\n* For Donors:\n\nAvailability of enough tissue for histological, immunohistochemical and RT-PCR analysis.\n\n* HOPE performed on the liver graft prior to transplantation.\n* For Recipients:\n* Availability of enough tissue for histological, immunohistochemical and RT-PCR analysis.\n* Age greater than\u002Fequal to 18 years.\n\nExclusion Criteria:\n\n* None","ALL","18 Years",{"count":69,"type":70},200,"ESTIMATED","INTERVENTIONAL",[73],"NA","Despite the good clinical results in terms of outcome with the application of HOPE prior to liver transplantation, very little is still known about the phenotypic and molecular changes that occur during perfusion\u002Fpreservation during HOPE, particularly with regard to endothelial trophism and activation. Delving into these aspects would be very useful to allow personalised perfusion treatments in the future, thus improving the outcome of transplantation.",[76,77],"Hepatic Transplantation","Rejection; Transplant, Liver",[79,80],"Transplant","Liver","2024-12-01",{"date":83,"type":84},"2024-12-05","ACTUAL",{"date":86,"type":84},"2023-01-17",{"date":88,"type":70},"2028-01",{"name":5,"class":6},1]