Commercial decalcified bone scaffolds were combined with autologous bone marrow mesenchymal stem cells to form tissue engineered bone in vitro to explore the effect of this tissue engineered bone in the clinical repair of long tubular bone defects in limbs.
Age: 18-55 years old, gender unrestricted;
Defective nonunion with bone loss exceeding 30% of the bone cross-section and a length of 2-3 cm;
Solitary bone cysts involving more than 50% of the bone cross-section.
Combined with local tumors, tuberculosis, infections or other bone-healing affecting lesions;
Systemic diseases affecting bone healing such as osteofibrous dysplasia, osteogenesis imperfecta, bone dystrophic diseases, and metabolic bone disorders;
Moderate-to-severe osteoporosis (BMD: T-score < -2.5);
Severe systemic conditions including systemic malnutrition, systemic infectious diseases, severe cardiopulmonary diseases, coagulation disorders, and major organ failure;