Purpose:
Carbapenemase-producing Enterobacterales (CPE) are a growing cause of healthcare-associated infections, linked to high morbidity, mortality, and cost. Current screening methods rely mainly on culture, which can take up to 48 hours and delay infection control actions.
This study aims to evaluate the real-life impact of implementing a rapid PCR-based algorithm for CPE detection compared with the standard culture-based protocol, focusing on time differences in isolation and de-isolation decisions in hospitalized patients.
Design:
A quasi-experimental, before-and-after, retrospective study conducted at Hospital Italiano de Buenos Aires (HIBA).
Primary Outcome:
Time (in hours) between rectal swab request and change in isolation status (application or removal of isolation label) before and after PCR implementation.
Population:
Adult patients (≥18 years) admitted between October 2023-April 2024 (pre-intervention) and October 2024-April 2025 (post-intervention), who had contact isolation initiated or discontinued based on CPE surveillance results.
Rationale:
The introduction of rapid molecular testing could reduce operational delays and unnecessary isolation days, optimizing resource use in a setting with high CPE endemicity.
Adult patients aged ≥18 years
Patients screened for carbapenemase-producing Enterobacterales (CPE) carriage by perianal swab within the first 5 days of hospital admission
Patients newly identified as CPE-colonized, leading to initiation of contact isolation
Patients found to be decolonized, leading to discontinuation of contact isolation
Lost samples
Insufficient samples
Invalid laboratory test results requiring repeat sampling