The MIRACLE study aims to investigate age-related mitochondrial dysfunction, mitochondrial RNA (mtRNA) release, inflammation, and cellular senescence in adult participants across three age groups. Skin-derived fibroblasts and peripheral blood mononuclear cells (PBMCs) will be isolated from skin biopsy and blood samples to characterize age-related cellular and molecular changes and to test experimental therapeutic strategies identified in preclinical studies. Serum, plasma, and whole-blood RNA will be used for protocol-defined analyses of circulating inflammatory mediators and systemic transcriptional signatures related to inflammation, type I interferon activation, mitochondrial stress response, immune aging, and senescence-associated pathways.
Male and female
Age between 18 and 90 years (stratified in three groups: young, middle-aged, elderly)
Written informed consent
Inability to understand the potential risk and benefits of the study
Legal incapacity
Subjects who have taken antibiotics, anti-inflammatory drugs, or antihistamines within the past 7 days
Diagnosis of diabetes mellitus