The purpose of this study is to assess the optimal protein intake to maximize whole-body protein net balance in healthy adults. In a randomized, double-blinded, crossover design, 12 young, healthy adult males and females aged 18-35 years, will consume a test diet consisting of free amino acid mixtures based on milk protein that vary in protein content 7 separate acute metabolic trials. The main study endpoint is optimal protein intake based on the lowest protein intake that maximizes whole-body protein net balance.
Aged between 18-35 years
BMI between 18.5-30 kg/m2
Healthy, recreationally active (exercise at least once per two weeks and a maximum of four days per week)
No physical limitations (i.e., able to perform all activities associated with daily living independently)
Smoking
Diagnosed musculoskeletal disorders
Use of any medications known to affect protein metabolism (i.e., corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications)
Chronic use of gastric acid-suppressing medication or anti-coagulants
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Treatment groupsSee each treatment group below.