The Effect of Repeated Whole-body NMES Sessions With or Without Protein Intake on Muscle Protein Synthesis During 3 Days of Bed Rest in Healthy Young Volunteers

The goal of this clinical trial is to examine the effect of whole-body electrostimulation with protein intake on muscle protein synthesis, muscle mass and function during bed rest in healthy young adults (18-35 years). The main questions to answer are:

* Does whole-body NMES followed by protein intake improve muscle protein synthesis rates during 3 days of bed rest? * Does repeated NMES sessions offer protective effects on muscle mass and function during extended periods of inactivity?

Researchers will compare sham electrical electrostimulation, electrical stimulation and electrical stimulation + protein groups to see if whole-body electrostimulation combined with protein intake offers the greatest improvement in muscle protein metabolism and muscle preservation.

Participants will:

* Undergo 3 days of bed rest while receiving one of the following interventions:

* Sham-NMES followed by standard nutrition (CON) * Whole-body NMES followed by standard nutrition(NMES) * Whole-body NMES followed by a bolus of 20g protein (NMES+PRO) * Receive heavy water (D2O) to assess body water turnover. * Undergo leg extension exercises to assess muscle function. * Have quadriceps muscle thickness measured via ultrasound. * Provide saliva samples for analysis. * Have calf circumference measured to monitor changes in muscle mass.